Figure 12.

Multiplex ABC-CRISPR of Ox1r and Ox2r causes chocolate-induced narcolepsy episodes. A, Schematic showing the two sets of sgRNA target sites within exons 2 and 3 of Ox1r gene and exons 3 and 5 of Ox2r gene. B, Quantification of Ox1r and Ox2r transcript levels in the ABC-NT and ABC-Ox1r/Ox2r^DKO mouse brains by real-time qRT-PCR. C, Quantification of the number of narcolepsy episodes in 3 ABC-Ox1r/Ox2r^DKO mice before and after chocolate feeding for 3 d. D, Representative hypnograms (ZT12-15) of ABC-Ox1r/Ox2r^DKO mice before and after chocolate feeding. Red triangles represent abnormal wake to REM transitions during narcolepsy-like episodes. E, Representative EEG/EMG signals depicting the abnormal wake to REM transition during one narcoleptic episode in the ABC-Ox1r/Ox2r^DKO mice. Data are mean ± SEM. B, C, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired t test.