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. 2022 Jul 13;42(28):5617–5640. doi: 10.1523/JNEUROSCI.0089-22.2022

Figure 7.

Figure 7.

Inducible ABC-expression of CREBVP16 or CRTC1CA causes significant sleep phenotypes. A, Schematic of Tet-on inducible (i)ABC-expression system with or without Dox. B, A flowchart of the iABC-expression and EEG/EMG sleep recording experiment. C, Immunoblotting of whole-brain lysates from iABC-eGFP mice before and after Dox treatment with anti-GFP and anti-ACTIN antibodies. D, Coimmunostaining of HA+ (red) and NeuN+ (green) neurons in the cortex and thalamus of the inducible (i)ABC-eGFP, ABC-CREBVP16, and ABC-CRTC1CA mice. E, Quantification of the viral transduction rates, which is calculated by the percentage of NeuN+ neurons that express HA-tagged proteins, in the cortical and thalamic neurons showed in D. F–I, Hourly plots of Wake time (F), REMS time (G), NREMS time (H), and EEG power spectra analysis of NREMS (I) of the iABC-eGFP mice (n = 8). J–M, Hourly plots of Wake time (J), REMS time (K), NREMS time (L), and EEG power spectra analysis of NREMS (M) in the iABC-CREBVP16 mice (n = 7). N–Q, Hourly plots of Wake time (N), REMS time (O), NREMS time, (P) and EEG power spectra analysis of NREMS (Q) in the iABC-CRTC1CA mice (n = 7). Data are mean ± SEM. F–Q, Two-way ANOVA with Dunn's multiple comparisons test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.