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. 2022 Jul 13;42(28):5617–5640. doi: 10.1523/JNEUROSCI.0089-22.2022

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Copyright © 2022 Wang, Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 8. — ABC-KO of Creb1 by Cre-loxP recombination causes hypersomnia. A, Schematic of ABC-KO of Creb1 by AAV-hSyn-Cre injection of Creb1^flox/flox mice. B, Immunoblotting of brain lysates from AAV-hSyn-mCherry or AAV-hSyn-Cre injected Creb1^flox/flox mice with anti-CREB and anti-ACTIN antibodies. C, Quantification of CREB expression in B (n = 4). D–F, Hourly plot of NREMS time (D), quantification of total NREMS time (E), and hourly plot of NREMS δ power (F) in the AAV-hSyn-mCherry (n = 9) or AAV-hSyn-Cre (n = 14) injected Creb1^flox/flox mice. G–K, Hourly plots of Wake (G) or REMS (I) time, quantification of total Wake (H) or REMS (J) time, and EEG power spectra analysis of NREMS, REMS, and Wake states (K) in the AAV-hSyn-mCherry (n = 9) or AAV-hSyn-Cre (n = 14) injected Creb1^flox/flox mice. Data are mean ± SEM. C, Unpaired t test. D–K, Two-way ANOVA with Dunn's multiple comparisons test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.