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. 2022 Jul 13;42(28):5617–5640. doi: 10.1523/JNEUROSCI.0089-22.2022

Figure 9.

Figure 9.

ABC-KO of exon 13 of Sik3 by Cre-loxP recombination causes hypersomnia. A, Schematic of ABC-KO of exon 13 of Sik3 by AAV-hSyn-Cre injection of Sik3-E13flox/flox mice. B, Immunoblotting of brain lysates from AAV-hSyn-mCherry or AAV-hSyn-Cre injected Sik3-E13flox/flox mice with anti-SIK3 and anti-ACTIN antibodies. C, Quantification of SLP-S expression in B (n = 4), which is calculated by the percentage of SLP-S/(SIK3-S+SLP-S). D–F, Hourly plot of NREMS time (D), quantification of total NREMS time (E), and hourly plot of NREMS δ power (F) in the AAV-hSyn-mCherry (n = 8) or AAV-hSyn-Cre (n = 8) injected Sik3-E13flox/flox mice. G–K, Hourly plots of Wake (G) or REMS (I) time, quantification of total Wake (H) or REMS (J) time, and EEG power spectra analysis of NREMS, REMS, and Wake states (K) in the AAV-hSyn-mCherry (n = 8) or AAV-hSyn-Cre (n = 8) injected Sik3-E13flox/flox adult mice. Data are mean ± SEM. C, Unpaired t test. D–K, Two-way ANOVA with Dunn's multiple comparisons test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.