KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-SKAP | Origene | TA333584 |
| Rabbit anti-SKAP | I. Cheeseman lab | 55 |
| Mouse anti-α-tubulin (DM1A) | Sigma-Aldrich | T6199 |
| Rabbit anti-Kif18A | Bethyl | A301–080A-M |
| Rabbit anti-MCAK | cytoskeleton | AKIN05 |
| Rabbit anti-p-Hec1-S69 | J. DeLuca lab | 25 |
| Human anti-CREST | Antibodies Incorporated | 15–234-0001 |
| Mouse anti-Hec1 | Abcam | ab3613 |
| Rat anti-α-tubulin | Bio-rad | MCA77G |
| Goat anti-mouse IgG Alexa Fluor 405, 488 and 568 | Invitrogen | A31553, A11001 and A11004 |
| Goat anti-rabbit IgG Alexa Fluor 405 and 568 | Invitrogen | A31556 and A11011 |
| Goat anti-rat IgG Alexa Fluor 488 | Invitrogen | A11006 |
| Goat anti-human IgG Alexa Fluor 568 | Invitrogen | A21090 |
| Chemicals, peptides, and recombinant proteins | ||
| Nocodazole | Sigma-Aldrich | M1404–50MG |
| MG132 | EMB Millipore | 474790–5MG |
| SiR-tubulin | Cytoskeleton | CY-SC002 |
| Experimental models: Cell lines | ||
| Rpe1-GFP cells | A. Khodjakov lab | 40 |
| Oligonucleotides | ||
| siRNA SKAP: 5′-AGGCUACAAACCACUGAGUAA-3′ | Sigma-Aldrich | 31 |
| siRNA SKAP2: 5′-GAAAGAGUCCGAUUCCUAG-3′ | Sigma-Aldrich | 30 |
| siRNA luciferase: 5’ CGUACGCGGAAUACUUCGA 3’ | Sigma-Aldrich | WD01818022 |
| Software and algorithms | ||
| FIJI (Version 2.3.0/1.53f) | ImageJ | 57 |
| MtrackJ | FIJI | 58 |
| Pyton code for kinetochore oscillations analysis | This paper | https://github.com/miquelrosassalvans/kinetochore-oscillations-analysis.git |
| Other | ||
| lipofectamine siRNAmax | Thermo Fisher Scientific | 13778075 |
| ProLong Gold Antifade reagent | Thermo Fisher Scientific | P36934 |