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. 2022 Jul 19;18(7):e1010306. doi: 10.1371/journal.pgen.1010306

Fig 1. A restriction enzyme-based method to enrich and purify centromeric DNA from human cells.

Fig 1

A. Schematic representation of the experimental design. B. Predicted distribution of the percentage of centromeric fragments in the indicated size bins after in silico digestion of the reference T2T-CHM13v1.0 genome with the SNE enzyme combination. Y-axis represents the percentage of centromeric fragments over total fragments in each length range. C. Distribution of centromeric base-pairs according to predicted fragment length after in silico digestion of the reference T2T-CHM13v1.0 genome with the SNE or SEB enzyme combinations. The y-axis on the left represents the percentage of centromeric base-pairs over total base-pairs in each length range. The dotted line at 2.8% represents the percentage of centromeric base-pairs in the reference genome, corresponding to the expected fraction of centromeric DNA in a theoretical non-enriched sample. The y-axis on the right reports the fold enrichment in centromeric base-pairs over the non-enriched sample (~2.8% of centromeric base-pairs in the reference genome).