(A) TSH concentrations in PD16 male and female offspring. Individual data points shown with mean and SEM whiskers. n = 7–8. (B) Thyroid gland weights in female offspring PD16, male PD27 and dams PD27. Females: n = 19–21, males: n = 15–17, Dams: n = 19–21. (C) Vacuolation of thyroid gland follicular colloid in male PD16 offspring. Left: incidences of vacuolation and severity, number of assessed male pups shown inside bars. Upper right: Thyroid gland with no vacuolation of follicular colloid. Lower right: thyroid gland with minimal vacuolation (arrows) of follicular colloid. *p< 0.05. Scalebar = 50 μm. PBDE: polybrominated diphenyl ethers (DE-71), PD: postnatal day, TSH: thyroid-stimulating-hormone.