Table 2. Ranking of candidate reference genes in zebrafish gut in wild-type and lepr mutant adults, under different feeding conditions.
BestKeeper | geNorm | NormFinder | |||||
---|---|---|---|---|---|---|---|
Ranking | r values | Ranking | SD values | Ranking | M values | Ranking | S values |
tmem50a | 0.928 | ef1a | 0.461 | tmem50a | 0.623 | tmem50a | 0.186 |
b2m | 0.892 | tmem50a | 0.624 | b2m | 0.674 | b2m | 0.271 |
rpl13 | 0.882 | actb2 | 0.626 | ef1a | 0.707 | ef1a | 0.276 |
ube2a | 0.857 | b2m | 0.647 | rpl13 | 0.732 | rpl13 | 0.298 |
ef1a | 0.823 | rpl13 | 0.709 | actb2 | 0.733 | actb2 | 0.304 |
rplp0 | 0.819 | p6gd | 0.717 | ube2a | 0.783 | p6gd | 0.315 |
actb2 | 0.812 | ube2a | 0.799 | p6gd | 0.796 | ube2a | 0.359 |
p6gd | 0.793 | rplp0 | 0.944 | rplp0 | 0.998 | rplp0 | 0.401 |
SD: standard deviation, r: Pearson product-moment correlation coefficient, SV: stability value, M: mean value of stability.