Table 2.

Relationship between spider mite fitness proxies and microbiome community structure, using Procrustes and Mantel tests.

	Fecundity						Longevity						Fecundity and longevity
	Bean		Cucumber		Tomato		Bean		Cucumber		Tomato		Bean		Cucumber		Tomato
	st.	p-v.	st.	p-v.	st.	p-v.	st.	p-v.	st.	p-v.	st.	p-v.	st.	p-v.	st.	p-v.	st.	p-v.
Euclidian	0.62	0.118	0.46	0.006	0.47	0.031	0.741	0.798	0.73	0.462	0.52	0.074	0.58	0.125	0.46	0.006	0.42	0.024
	0.61	0.080	0.45	0.001	0.48	0.028	0.721	0.686	0.70	0.196	0.46	0.013	0.57	0.104	0.44	0.001	0.41	0.007
	0.62	0.087	0.49	0.003	0.49	0.014	0.734	0.849	0.71	0.278	0.48	0.021	0.57	0.095	0.49	0.003	0.43	0.001
	0.63	0.092	0.51	0.005	0.48	0.013	0.735	0.880	0.70	0.225	0.49	0.029	0.58	0.102	0.51	0.004	0.42	0.007
	0.62	0.088	0.54	0.017	0.45	0.004	0.711	0.596	0.71	0.265	0.49	0.026	0.58	0.106	0.53	0.017	0.39	0.007
Manhattan	0.62	0.118	0.46	0.006	0.47	0.031	0.741	0.798	0.73	0.462	0.52	0.074	0.55	0.143	0.44	0.007	0.37	0.017
	0.61	0.080	0.45	0.001	0.48	0.028	0.721	0.686	0.70	0.196	0.46	0.013	0.54	0.115	0.42	0.001	0.36	0.007
	0.62	0.087	0.49	0.003	0.49	0.014	0.734	0.849	0.71	0.278	0.48	0.021	0.54	0.120	0.47	0.003	0.37	0.001
	0.63	0.092	0.51	0.005	0.48	0.013	0.735	0.880	0.70	0.225	0.49	0.029	0.55	0.120	0.49	0.003	0.36	0.007
	0.62	0.088	0.54	0.017	0.45	0.004	0.711	0.596	0.71	0.265	0.49	0.026	0.54	0.109	0.51	0.015	0.33	0.007
Euclidian	0.28	0.105	0.57	0.014	0.56	0.019	−0.133	0.758	0.03	0.462	0.31	0.146	0.27	0.106	0.57	0.014	0.60	0.010
	0.34	0.072	0.73	0.002	0.45	0.026	−0.118	0.719	0.22	0.110	0.64	0.025	0.34	0.081	0.73	0.002	0.53	0.007
	0.32	0.077	0.71	0.004	0.41	0.018	−0.089	0.662	0.17	0.205	0.43	0.081	0.34	0.071	0.71	0.004	0.45	0.001
	0.31	0.076	0.64	0.008	0.51	0.015	−0.185	0.849	0.24	0.114	0.49	0.058	0.30	0.084	0.64	0.008	0.57	0.004
	0.29	0.090	0.49	0.029	0.61	0.008	−0.084	0.640	0.17	0.224	0.42	0.100	0.28	0.098	0.49	0.028	0.66	0.008
Manhattan	0.28	0.105	0.57	0.014	0.56	0.019	−0.133	0.758	0.03	0.462	0.31	0.146	0.26	0.115	0.57	0.014	0.64	0.010
	0.34	0.072	0.73	0.002	0.45	0.026	−0.118	0.719	0.22	0.110	0.64	0.025	0.33	0.085	0.74	0.002	0.60	0.004
	0.32	0.077	0.71	0.004	0.41	0.018	−0.089	0.662	0.17	0.205	0.43	0.081	0.31	0.088	0.72	0.003	0.52	0.001
	0.31	0.076	0.64	0.008	0.51	0.015	−0.185	0.849	0.24	0.114	0.49	0.058	0.28	0.091	0.65	0.006	0.63	0.004
	0.29	0.090	0.49	0.029	0.61	0.008	−0.084	0.640	0.17	0.224	0.42	0.100	0.28	0.099	0.50	0.026	0.71	0.008

The distance matrices used for both the Procrustes and the Mantel test measure the differences between the microbiome composition of the different spider mite lines on their host plant (bean, cucumber, or tomato) and the differences between the fecundity, longevity, or both fecundity and longevity at the last time point (150 days). The significant results (p-value lower than 0.05) are visualized in bold. For the microbiome distance table, the unweighted UniFrac was chosen, while for the performance table two different distance measures were used (i.e., Euclidean and Manhattan). The Mantel test was done with 9,999 permutations based on the Pearson method.