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. 2022 Jul 5;12:936145. doi: 10.3389/fonc.2022.936145

Figure 3.

Figure 3

Effect of absence of GH on response of syngeneic mouse melanoma tumors to cisplatin treatment in vivo. (A) Mouse Fluc-B16-F10 cells were grafted intradermally on the right flank of syngeneic C57BL6/J wild-type (WT) and GH knockout (GHKO) mice (n=6). The changes in tumor volume (Fluc-B16-F10 in WT and GHKO mice) from digital caliper measurement and representative tumors post-dissection (A) and tumor mass (B) corroborate suppressed tumor growth in GHKO mice but not improved tumoral response to cisplatin in the GHKO mice. Western-blot assessment of GH downstream signaling and ABC transporter protein levels (C) and qPCR analysis of ABC transporter RNA expression involved in multi-drug efflux from the tumors in WT and GHKO mice (D) are shown. (*p < 0.05, mouse studies – repeated measure using SPSS; other assays - Students t test, n = 3). (E) Spearman correlation analysis for transcript levels of GHR and ABC transporter and IGF1R and ABC transporters in 471 human melanoma patients in the TCGA cohort (generated using Linkedomics).