Table 1.
Four differentially expressed proteins identified in aluminum ion treated SH-SY5Y cells.
| Accession No.a | Protein name | MW (Da) | Mascot score | Match queries | pI | Sequence coverage | Peptide | Protein function |
|---|---|---|---|---|---|---|---|---|
| O43310 | CBP80/20-dependent translation initiation factor | 67,544 | 29 | 6 | 6.10 | 16% | R.NNSSDVDTK.L + 2 Deamidated (NQ) R.SSTGEPFR.V + 2 Phospho (ST) R.DKMLCPSESMLTR.S + Oxidation (M); Phospho (ST) - MENSSAASASSEAGSSR.S + Deamidated (NQ); Oxidation (M); 2 Phospho (ST) M.ENSSAASASSEAGSSR.S + Deamidated (NQ); 6 Phospho (ST) - MENSSAASASSEAGSSR.S + Deamidated (NQ); 5 Phospho (ST) |
Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD). |
| Q15075 | Early endosome antigen 1 | 162,367 | 21 | 4 | 5.5 | 4% | M.LRRILQR.T + Deamidated (NQ) R.ENQSLQIKHTQALNRK.W + Deamidated (NQ) R.ENQSLQIKHTQALNRK.W + 4 Deamidated (NQ) K.MEKEALMTELSTVKDK.L + Oxidation (M); 2 Phospho (ST) |
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. |
| Q8WUT4 | Leucine-rich repeat neuronal protein 4 | 78,794 | 34 | 3 | 6.82 | 6% | R.AFACFPALQLLNLSCTALGR.G + 2 Carbamidomethyl (C); 2 Deamidated (NQ) R.STYAQGTTVAPSAAPATRPAGDQQSVSK.A + 6 Phospho (ST) R.QTLPLLLLTVLRPSWADPPQEK.V |
May play an important role in hippocampus-dependent long-lasting memory |
| O00459 | Phosphatidylinositol 3-kinase regulatory subunit beta | 81,574 | 35 | 6 | 6.2 | 16% | R.LKSRIAEIHESR.T + 2 Phospho (ST) R.APALGPAVRALGATFGPLLLR.A + Phospho (ST) R.TWYVGKINRTQAEEMLSGK.R R.TWYVGKINRTQAEEMLSGK.R + Deamidated (NQ) R.TWYVGKINRTQAEEMLSGK.R + 2 Deamidated (NQ) K.IRDQYLVWLTQKGAR.Q + 2 Deamidated (NQ); Phospho (Y) |
Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. |
a
Swiss-Prot/TrEMBL accession number was given from https://world-2dpage.expasy.org/swiss-2dpage/.