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. 2019 Jan 2;27(2):531–541. doi: 10.1016/j.jfda.2018.11.007

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© 2019 Taiwan Food and Drug Administration

This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Mass and charge of Lys-C + trypsin + Glu-C-digested peptide with the three disulfides (Cys35-Cys53, Cys56-Cys71, and Cys74-Cys88), for Enbrel® (A) and TuNEX® (B). The sequence and theoretical mass of the peptide along with the observed monoisotopic (1st isotopic) mass are indicated in the insert. CID-MS² spectrum of the precursor for Enbrel® (C) and TuNEX® (D). CID-MS² spectrum of the precursor but with a different charge state (3+) from Figure 2A, B for Enbrel® (E) and TuNEX® (F). ETD-MS² spectrum of the precursor for Enbrel® (G) and TuNEX®(H).

Fig. 2 — Mass and charge of Lys-C + trypsin + Glu-C-digested peptide with the three disulfides (Cys35-Cys53, Cys56-Cys71, and Cys74-Cys88), for Enbrel® (A) and TuNEX® (B). The sequence and theoretical mass of the peptide along with the observed monoisotopic (1st isotopic) mass are indicated in the insert. CID-MS² spectrum of the precursor for Enbrel® (C) and TuNEX® (D). CID-MS² spectrum of the precursor but with a different charge state (3+) from Figure 2A, B for Enbrel® (E) and TuNEX® (F). ETD-MS² spectrum of the precursor for Enbrel® (G) and TuNEX®(H).