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. 2019 Jan 2;27(2):531–541. doi: 10.1016/j.jfda.2018.11.007

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© 2019 Taiwan Food and Drug Administration

This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Mass and charge of Lys-C + trypsin + Glu-C-digested peptide with the two disulfides (Cys78-Cys96 and Cys98-Cys104), for Enbrel® (A) and TuNEX® (B). The sequence and theoretical mass of the peptide along with the observed monoisotopic (1st isotopic) mass are indicated in the insert. CID-MS² spectrum of the precursor for Enbrel® (C) and TuNEX® (D). ETD-MS² spectrum (using Orbitrap) of the precursor for Enbrel® (E) and TuNEX®(F). CID-MS³ spectrum of the precursor from Figure 3E (m/z 680.27) for Enbrel®(G) and Figure 3F (m/z 680.27) for TuNEX®(H).

Fig. 3 — Mass and charge of Lys-C + trypsin + Glu-C-digested peptide with the two disulfides (Cys78-Cys96 and Cys98-Cys104), for Enbrel® (A) and TuNEX® (B). The sequence and theoretical mass of the peptide along with the observed monoisotopic (1st isotopic) mass are indicated in the insert. CID-MS² spectrum of the precursor for Enbrel® (C) and TuNEX® (D). ETD-MS² spectrum (using Orbitrap) of the precursor for Enbrel® (E) and TuNEX®(F). CID-MS³ spectrum of the precursor from Figure 3E (m/z 680.27) for Enbrel®(G) and Figure 3F (m/z 680.27) for TuNEX®(H).