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Workflow for targeting amine- and phenol-containing metabolites in bladder cancer urine using dansylation isotope labeling and LC-MS. Commercial standard compounds were labeled with 13C2-DnsCl, spiked with 12C2-DnsCl–labeled urinary metabolites, and then analyzed by LC-FT/MS and LC-HCT/MS. The blue block on the right represents serial dilutions used to prepare standard curves in LC-HCT/MS.
