Table 1.
Parameters of multiple reaction monitoring (MRM) for targeted peptide/glycopeptide analysis.
| Compound name | FVd (V) | CEe (V) | Precursor ion (m/z) | Product ion (m/z) |
|---|---|---|---|---|
| H3N4F1-IgG1a | 135 | 15 | 878.8 | 204.1 |
| H3N4F1-IgG2b | 135 | 15 | 868.1 | 204.1 |
| H3N4F1-IgG3/4c | 135 | 15 | 873.4 | 204.1 |
| H3N5F1-IgG1 | 135 | 15 | 946.5 | 204.1 |
| H3N5F1-IgG2 | 135 | 15 | 935.8 | 204.1 |
| H3N5F1-IgG3/4 | 135 | 15 | 941.1 | 204.1 |
| H4N4F1-IgG1 | 135 | 15 | 932.8 | 204.1 |
| H4N4F1-IgG2 | 135 | 15 | 922.1 | 204.1 |
| H4N4F1-IgG3/4 | 135 | 15 | 927.4 | 204.1 |
| H4N4F1S1-IgG1 | 135 | 15 | 1029.8 | 204.1 |
| H4N4F1S1-IgG2 | 135 | 15 | 1019.1 | 204.1 |
| H4N4-IgG1 | 135 | 15 | 884.1 | 204.1 |
| H4N4-IgG2 | 135 | 15 | 873.4 | 204.1 |
| H4N5F1-IgG1 | 135 | 15 | 1000.5 | 204.1 |
| H4N5F1-IgG2 | 135 | 15 | 989.9 | 204.1 |
| H5N4F1-IgG2 | 135 | 15 | 976.1 | 366.1 |
| H4N5F1-IgG3/4 | 135 | 15 | 995.1 | 204.1 |
| H4N5-IgG1 | 135 | 15 | 951.7 | 204.1 |
| H4N5-IgG2 | 135 | 15 | 941.1 | 204.1 |
| H5N4F1-IgG1 | 135 | 15 | 986.8 | 366.1 |
| H5N4F1S1-IgG1 | 135 | 15 | 1083.8 | 366.1 |
| H5N4F1S1-IgG2 | 135 | 15 | 1073.1 | 366.1 |
| H5N4F1S1-IgG3/4 | 135 | 15 | 1078.4 | 366.1 |
| H5N4-IgG1 | 135 | 15 | 938.1 | 366.1 |
| H5N5F1-IgG1 | 135 | 15 | 1054.5 | 366.1 |
| H5N5F1-IgG2 | 135 | 15 | 1043.8 | 366.1 |
| IgG (DTLMISR) | 135 | 9 | 418.2 | 619.4 |
| 9 | 418.2 | 506.3 | ||
| IgG1 (GPSVFPLAPSSK) | 135 | 20 | 593.8 | 846.5 |
| 20 | 593.8 | 699.4 | ||
| IgG2 (GLPAPIEK) | 135 | 15 | 412.7 | 654.4 |
| 15 | 412.7 | 486.3 | ||
| IgG3 (WYVDGVEVHNAK) | 135 | 6 | 472.9 | 697.4 |
| 6 | 472.9 | 534.3 | ||
| IgG4 (TTPPVLDSDGSFFLYSR) | 135 | 9 | 635.0 | 425.2 |
| 15 | 951.6 | 850.6 |
a
IgG1 glycopeptide: glycan-EEQYNSTYR; H, hexose; N, N-acetylglucosamine; F, fucose; S, N-acetylneuraminic acid.
b
IgG2 glycopeptide: glycan-EEQFNSTFR.
c
IgG3/4 glycopeptide: glycan-EEQYNSTFR (IgG3); glycan-EEQFNSTYR (IgG4).
d
FV: fragmentor voltage.
e
CE: collision energy.