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. 2022 Jul 5;12:828439. doi: 10.3389/fcimb.2022.828439

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Copyright © 2022 Madhvi, Mishra, Chegou and Baker

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of in vitro CFUs of M. bovis BCG after overexpression of IFITs at 12-hours and 96-hours (A); and knock-down of IFITs at 12-hours and 96-hours (B). Compared to M. bovis BCG, we observed significant reduction of CFUs after overexpression of IFITs and significant increase of CFUs after knock-down of IFITs. The results indicate the association of IFITs with in vitro survival of Mycobacteria. BCG, Bacillus Calmette-Guerin; IFIT, interferon-induced protein with tetratricopeptide. Table 2 depicts the %-increase or decrease in CFUs after in vitro knock-down and overexpression respectively. ***p<0.001.