Fig. 8.

Hypoxia treatment alters the post-translational modification of APP. A Western blot analysis of BACE1 levels from 4 mo wild-type (WT) and APP/PSEN1 mice treated in normoxia or hypoxia for 6 weeks. B Quantification of the relative BACE1 relative protein levels in cortex and hippocampus of 4 mo APP/PSEN1 mice. C qPCR analysis of the Bace1 mRNA levels in cortex and hippocampus of 4 mo APP/PSEN1 mice. D Western blot analysis of the levels of C-terminal factors (CTFs) of APP from 4 mo APP/PSEN1 mice. E Quantification of the relative CTF levels in cortex and hippocampus of 4 mo mice. F Ratio of APP CTFs in cortex and hippocampus of 4 mo APP/PSEN1 mice. Data are means ± SEM. *P < 0.05, ***P < 0.005 in T-test. n = 8 APP/PSEN1 N 4 mo, n = 9–10 APP/PSEN1 H 4 mo. CTF C-terminal factor, hippo hippocampus, H hypoxia, mo months old, N normoxia