TABLE 1.

Oligonucleotide sequences used in this study

Primer	Sequence (5′ to 3′)	Saccharomyces cerevisiae positions	Specificity	Reference
Set Aa
Euk1A	CTGGTTGATCCTGCCAG	4 to 20	Eukarya	48
Euk516r-GCb	ACCAGACTTGCCCTCC	563 to 548	Eukarya	2
Set B
Euk1209f-GCc	CAGGTCTGTGATGCCC	1423 to 1438	Eukarya	14
Uni1392r	ACGGGCGGTGTGTRC	1641 to 1627	Universal	22
Set C
EukA	AACCTGGTTGATCCTGCCAGT	1 to 21	Eukarya	33
EukB	TGATCCTTCTGCAGGTTCACCTAC	1795 to 1772	Eukarya	33

^aSet A was also used for T-RFLP analysis, using hexachlorofluorescein-labeled Euk1A and Euk516r without the GC clamp. 

^bThe GC clamp sequence is CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGG. 

^cThe GC clamp sequence is CGCGCGCCGCGCCCCGCGCCCGTCCCGCCGCCCCCGCCCG.