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. 2022 Apr 8;127(2):364–376. doi: 10.1038/s41416-022-01800-y

Fig. 4. xCT and GPX4 expression levels decreased in PLB-induced ferroptosis.

Fig. 4

a Representative western blot results of ferroptosis-related protein expression levels in U251 and C6 cells treated with PLB. b Quantitation of western blot results of ferroptosis-related proteins. GAPDH served as the loading control (n = 4). c Representative immunohistochemical staining results of xCT and GPX4 in xenograft glioma tissues in nude mice treated with PLB or TMZ. Scale bar, 100 μm. d Statistical analysis of immunohistochemical staining results of xCT and GPX4 (n = 5). e mRNA expression levels of xCT and GPX4 in U251 (n = 4) and C6 cells (n = 5) treated with PLB. The results are expressed as mean value ± S.D. *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001 versus the control group.