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. 2022 Apr 8;127(2):364–376. doi: 10.1038/s41416-022-01800-y

Fig. 5. PLB induced GPX4-dependent cell death by causing GPX4 degradation.

Fig. 5

a, b Western blot and quantitation results of the GPX4 expression in GPX4 plasmid-transfected U87 (n = 4) and U251 cells (n = 5). c, d Inhibitory effects of PLB on GPX4 overexpressed and vector control U87 and U251 cells via the MTS assay (n = 4). e, f Cell viabilities of U251 and U87 cells treated with PLB in the presence of MG132 (2 μM) or Baf-A1 (100 nM) for 48 h (n = 5). g Coimmunoprecipitation results of GPX4 and HSC70 or Lamp-2a in GPX4-overexpressing U251 cells treated with 6 μM PLB in the presence of Baf-A1 (100 nM). Cell lysates were immunoprecipitated with anti-GPX4 antibody and subjected to western blot with the indicated antibodies. *P < 0.05 and **P < 0.01, versus the control group.