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. 2022 Jul 19;12:12306. doi: 10.1038/s41598-022-16391-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Optimization of S. cerevisiae SAGA cryo-EM specimen preparation. (a) Representative raw images of negatively stained TAP-purified and FLAG-purified SAGA. (b) Representative cryo-EM micrographs of SAGA vitrified on Quantifoil grids with continuous carbon support taken at a defocus value of 3 μm, C-flat carbon grids without a support layer at a 4.5 μm defocus, C-flat grids with carbon support at a 2.5 μm defocus and Lacey carbon grids with graphene oxide support layer at a defocus value of 2.5 μm.