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. 2022 Jul 19;12:12312. doi: 10.1038/s41598-022-16564-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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huH-1 is the most sensitive cell line to ERY974. (a) GPC3 expression level on the surface of HepG2, PC-10, and huH-1 cells. ABC for each cell line is shown. (b) The EC₁₀ values of cytotoxicity and T cell activation assay of ERY974 in HepG2, PC-10, and huH-1. The PBMCs from four independent donors were used as effector cells. EC₁₀ was calculated using the sigmoid Emax model. Data are shown as mean ± SD (n = 3). ABC means antigen-binding capacity.