TABLE 1.

Primers used for PCR-based cloning

Primer no.	Primer designation	Sequence (5′ to 3′)b	Position (bp)c
1	Tri5/p1	AGATCGTACAGCTAAATTCAGAGC	15500–15523
2	Tri5/p2	CCACTAGCTCAATTGAACTTAGGA	17393–17370
3	Tri6/p1	TGGAGNGCNTTNCCNCTNTTTGA	12663–12685
4	Tri6/p2	TTGTGTATNCGNCTATAGTGATC	13276–13254
5	Tri4/p2	TCNGTYTTNCCNGGDATNGC	9757–9776
5′	Tri4/2p2	GTCGATGTCGGCTTTAGTGGTGTC	9810–9833
6	Tri4/p1	GGNCCNTAYYTNGARTTYTA	11293–11274
6′	Tri4/2p1	AGCCTTAGTCAATATCCCCATCAG	11431–11409
7	Tri3/p1	CAGCGCTATACAGATGGAAGTC	6823–6844
8	Tri3/p2	TCGGTATAGTTTGCATCATTGTAG	8487–8464
9	Tri7/p1	CGCATCGAAAGTGGAAGGTT	4618–4637
9′	Tri7/p5	TGCCTCTCCTGCCATCGTC	4750–4768
10	Tri7/p2	ATGAACCAAAATGATACCCAGATG	5823–5800
11	Tri11/p2	ACATTNAGNGGNGGCTTGTTCC	21419–21440
12	Tri11/p1	ATGTTTCAATATTCNTTNTGGCC	23140–23118
13	Tri102/p2	TGGTGNGGNACATGATCNGTNAGG	23890–23913
14	Tri102/p1	GACCTNGAATCNCAGCCNGACGAC	25661–25638
15	Itri5n/p1	ACTTGACTAGGTAGCCACGGTGTTTTATTC	15723–15694
16	Itri5n/p2	GAAAAGGTCAAGCATCAGGATACAGAGGA	17204–17232
17	Itri65/p1	GAAGTCAAAGTCGAAGCAAACAAGTGG	12878–12852
18	Itri65/p2	GCCAAGCAAATGCCCGTATCCCAGTT	12971–12996
19	Itri7n/p1	GAAACCGCGATGACCGATACTGCTGCTAAG	4837–4808
20	Itri7n/p2	GCCACAAAGCAAGACGGAGACGAAGGAG	5744–5771
21	Itri11n/p2	TAGCCGCAATTGGTACCAGGATAGCAG	21481–21455
22	Itri11n/p1	CCGCAGGGGATGGAAAAAGAGGTTGTA	22979–23005
23	I2tri5/p2	GCGATGCTGCCAGGCTCAAGGTAAAG	14905–14880
24	I2tri5/p1	AACTAATTGTCTGTAACCTGAGCCTGTAACCA	17553–17584
25	Itri4nd/p2	CYGATTGGAGGAGAACACTTGATAACATTT	9936–9907
26	Itri4n/p1	GCTTTGGTCCCGGGATCTTTCGCAGTG	11305–11331
26′	Itri4d/p1	GGACGATGGGGCCTTGAGGATGTGATG	8425–8451d
27	Itri8/p1	TTAATTTAGGCGAACAAGAGACGGAAGACAGT	1191–1160
28	Itri8/p2	AGTATCGGTCATCGCGGTTTCATTCTGG	4817–4844
29	Itri102n/p5	ATTTTGCCGGCTTTGTCTGCATCCTTCTC	24716–24688
30	Itri102nd/p1	TGAAGAACAAAGTAGGTAGYGCAGACATT	25510–25538
31	Tri6nd/p2	CACGACCAGGAAGGACAACAATG	13197–13219
32	Tri6nd/p1	CTTGGCGGGATCGGGAGAC	12713–12695
33	Tri5n/p1	GGGGAGTTGTTAGGCTTGGATTTG	14358–14335
34	Itri3nd/p1	TCCGAAGAGAAGTAGCGATGAARAGAT	6857–6931
35	Itri3d/p2	TCCGTTGAGAGCATAGACTTTGTTGTGAA	8322–8350
36	Itri53/p3	TGTATCCTGATGCTTGACCTTTTC	17227–17204
37	Itri53/p2	CTTGCAGAACTGAACAGGCGACGGTATG	18282–18309
38	Tri8/delp2	ACAGCGTGGACACAGCAACCCTTACTT	4203–4229
39	I53n/np3	TCGGCAACATGGCAACGGACTT	20553–20574
40	I11n/np4	GCTTTTCGCGCTGTTGCCGTAGG	20764–20742
41	8n/p2	AGATGAATTACCAGGCAGATGTCCAGAAGTCG	2567–2598
42	7d/p1	AGGCCGTGAAAATGAACCGCTTGATGTA	1963–1936d
43	5d/np1	CACTACGAAACTGCCGGCCATCACATA	12866–12840d
44	7d/np1	TGTGCCGTGAGCCCCAGAATGAAGC	2271–2247d
45	I7d/p1	ACGGTGTACGAGTGGTTTGTGTCCTATTCA	2363–2392d
46	Itri102d/p4	TATCCCTCTTTCTCCTTGGTGACTACTGG	21988–22016d
47	Tri5d/p4	TGGTTTGGGTCATTGTGTTGGGGCTTCT	14063–14090d
48	I11d/np4	TGCTTTCATTAATTTTCGCTTTTC	17990–17967d
48′	I11d/np2	CTTGCAACTTTAACGTGTCCAGAT	20533–20556d
49	I4d/np3	ATGGCAAGTTATGGGGTCAGC	9187–9207d
50	Itri102nd/p2	GCTAATGTCTACTACTTCGCTATGGCACTGG	21122–21092d

^aShown in Fig. 1. 

^bR = A or G; Y = C or T; D = A or G or T; N = A or C or G or T. 

^cAccording to the deposited sequence (GenBank accession no. AF336365; from G. zeae 88-1). 

^dAccording to the deposited sequence (GenBank accession no. AF336366; from G. zeae H-11).