TABLE 2.
Sizes of Tri ORFs, introns, and noncoding regions in trichothecene biosynthesis gene clusters of G. zeae and F. sporotrichioides
| Region(s) | No. of nucleotides (bp)/amino acids
|
Putative intron(s) (bp)a
|
||||||
|---|---|---|---|---|---|---|---|---|
| 88-1 | H-11 | G. zeaeb | F. sporotrichioidesb | 88-1 | H-11 | G. zeaeb | F. sporotrichioidesb | |
| 8 | 1,335/445 | 1,338/446 | NAc | 1,344/447 | 0 | 0 | NA | 0 |
| 8–7 | 701/—d | 681/— | ||||||
| 7 | 1,377/442 | 1,444/431 | NA | 1,250/398 | 51 | 151 | NA | 53 |
| 7–3 | 799/— | 812/— | ||||||
| 3 | 1,763/512 | 1,768/512 | NA | 1,783/513 | 55, 49, 58, 65 | 56, 49, 58, 69 | NA | 56, 61, 59, 65e |
| 3–4 | 1,165/— | 946/— | ||||||
| 4 | 1,742/521 | 1,742/521 | NA | 1,743/520 | 58, 52, 69 | 58, 52, 69 | NA | 58, 52, 70e |
| 4–6 | 1,171/— | 1,168/— | ||||||
| 6 | 657/219 | 657/219 | 657/218 | 654/217 | 0 | 0 | 0 | 0 |
| 6–5 | 2,935/— | 2,886/— | ||||||
| 5 | 1,180/376 | 1,187/376 | 1,187/375 | 1,185/374 | 52 | 59 | 59 | 60 |
| 11 | 1,741/493 | 1,741/493 | 1,741/492 | 1,722/492 | 57, 54, 78, 73 | 57, 54, 78, 73 | 57, 55, 82, 68 | 49, 54, 76, 64 |
| 11–102 | 649/— | 632/— | ||||||
| 102 | 1,905/591 | 1,902/590 | 1,906/598 | 1,902/598 | 56, 76 | 56, 76 | 60, 76 | 56, 49e |
| 101 | 1,202/400f | 1,356/452 | 1,356/451 | 1,380/459 | 0 | 0 | 0 | 0 |
a
The order of introns is 5′ to 3′ in each ORF.
b
Sequences of noncoding DNA regions are not compared here. Refer to Table 3 for accession numbers of each sequence.
c
NA, not available.
d
—, no translation product from noncoding DNA regions.
e
Confirmed by cDNA sequencing.
f
Putative translation product from an incomplete ORF.