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. 2022 Jul 6;13:887916. doi: 10.3389/fimmu.2022.887916

Table 1.

Distribution of soluble immune checkpoint-related proteins in control, AIS and IAC patients.

  Control (N=35) AIS (N=43) IAC (N=81)           
      Early stage (N=50) Late stage (N=31)          
Marker Median (IQR) pg/ml Median (IQR) pg/ml Median (IQR) pg/ml Median (IQR) pg/ml *P **P ***P ****P P trend
sBTLA 280.52 (113.59-511.51) 228.01 (181.84-274.19) 211.52 (170.17-290.44) 244.28 (178.41-329.29) 0.68 0.55 0.98 0.25 0.98
sCD27 556.25 (466.54-681.61) 489.69 (399.67-629.86) 735.4 (476.1-1198.23) 1382.75 (943.44-1745.74) 2.24E-01 5.37E-05 1.05E-06 2.93E-04 7.80E-09
sCD28 64.51 (21.04-161.75) 31.77 (22.24-44.18) 29.67 (23.31-38.53) 49.74 (32.81-71.38) 0.19 0.29 0.73 0.14 0.84
sCD80 106.43 (67.27-137.99) 66.42 (43.94-91.73) 109.73 (67.42-341.77) 285.04 (90.3-398.26) 1.46E-02 9.74E-02 4.44E-05 0.03 5.00E-04
sCD137 177.01 (149.97-257.58) 65.11 (46.03-111.6) 132.06 (79.18-188.46) 176.76 (130.55-216.77) 2.85E-05 4.59E-02 2.30E-05 0.02 0.19
sCTLA4 7.81 (6.74-20.85) 5.19 (2.46-6.83) 5.91 (3.89-9.37) 9.63 (5.35-10.64) 0.03 0.30 0.14 0.25 0.93
sGITR 37.98 (16.02-68.48) 18.1 (9.7-71.37) 10.64 (6.92-26.63) 10.64 (6.85-17.99) 0.87 0.08 0.16 0.67 0.04
sHVEM 13.86 (8.98-22.35) 4.87 (2.59-17.23) 20.08 (14.71-29.34) 22.51 (16.71-41.88) 0.08 0.01 0.02 0.6 0.01
sIDO 6.68 (4.52-10.7) 5.89 (4.15-8.21) 6.88 (4.15-12.42) 8.96 (3.62-14.79) 0.37 0.76 0.2 0.64 0.51
sLAG3 12.97 (9.93-16.02) 15.09 (15.09-15.09) 14.63 (9.31-20.24) 10.66 (6.4-17.47) 1.00 0.93 0.33 0.37
sPD1 13.78 (9.35-24) 12.39 (7.2-16.06) 16.16 (11.23-22.43) 17.05 (12.01-20.95) 0.36 0.64 0.01 0.46 0.08
sPDL1 0.35 (0.32-0.45) 0.29 (0.24-0.37) 0.18 (0.11-0.29) 0.24 (0.13-0.29) 3.82E-03 3.63E-05 0.05 0.87 3.88E-05
sPDL2 268.82 (218.56-339.59) 321.21 (228.13-402.55) 467.19 (349.75-694.05) 702.54 (388.59-852.24) 0.21 3.65E-08 1.16E-06 0.07 1.99E-10
sTIM3 131.31 (113.25-155.83) 28.98 (17.84-56.54) 39.49 (16.45-66.41) 67.75 (28.78-156.22) 2.347E-03 0.04 0.33 0.11 0.96

AIS, adenocarcinoma in situ; IAC, invasive adenocarcinoma. Early stage indicates stage I&II disease, Late stage indicate stage III&IV disease, the staging criteria according to NCCN Clinical Practice Guidelines Non-small cell lung cancer v1, 2022 *P indicates Control vs. AIS, **P indicates Control vs. IAC, ***P indicates AIS vs. IAC, ****P indicates early stage vs. late stage, †P for trend was analyzed using Jonckheere-Terpstra test.