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. 2022 May 26;119(22):e2123202119. doi: 10.1073/pnas.2123202119

Fig. 2.

Fig. 2.

The loss of E12 in MEFs and mouse tissues leads to increased cellular senescence. (A) The level of Trp73α, Trp73α1, and Actb transcripts was measured in WT and E12+/− MEFs. (B) SA-β-galactosidase staining was performed with WT, E12+/-, and E12−/− MEFs. The percentage of SA-β-gal-positive cells was shown in each panel. (C) The level of PML, p130, p21, and actin proteins was measured in WT, E12+/-, and E12−/− MEFs. (D) Growth curves were performed with WT and E12−/− MEFs over 7 d. (E) The level of Trp73α, Trp73α1, and Actb transcripts was measured in liver and kidney tissues from age- and sex-matched WT and E12+/− mice (100 wk; Female). (F) qPCR was used to analyze relative Cdkn1a (p21) and Cdkn2a (p16) expression in liver and kidney tissues from age- and sex-matched WT and E12+/− mice (100 wk; Female). One-way ANOVA was used to calculate P values. *P < 0.05; **P < 0.01; ****P < 0.0001. (G) SA-β-galactosidase staining was performed with kidney tissues from age- and sex-matched WT and E12+/− mice (100 wk; Female).