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Results of label-free quantification in the discovery stage. (A) Proteomic workflow of label-free quantification. (B) Flowchart of verification process using data-independent acquisition approach. (C) Correlation of protein control/urothelial carcinoma fold changes between the discovery and verification cohorts. DEP, differentially expressed protein; FASP, filter-aided sample preparation; LC-DIA/MS, liquid-chromatography data independent acquisition mass spectrometry.
