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. Author manuscript; available in PMC: 2022 Jul 20.
Published in final edited form as: Nature. 2022 Jun 1;606(7915):739–746. doi: 10.1038/s41586-022-04760-8

Extended Data Figure 1. Distribution of cardiac-innervating neurons in the nucleus ambiguus (Amb).

Extended Data Figure 1.

a, Sagittal schematic view displaying locations of Amb neurons (circles) within a postnatal day 2 Amb nucleus. Map is an overlay showing the locations of Amb neurons from all sagittal sections spanning a single Amb nucleus. Green fill circles, cardiac Amb neurons retrograde labeled by cholera toxin B (CTB) injection into pericardial space (CTB>Heart). Grey fill circles, other Amb neurons. Note cardiac Amb neurons localize primarily to “external formation” of Amb, surrounding the principal rostral-caudal column of Amb neurons. b, Representative sagittal section of Amb with AmbCardiac neurons labeled in green by retrograde labeling by CTB injection in heart (CTB>Heart). Retrograde labeled cells localized to the Amb external formation. AmbC, nucleus ambiguus compact formation. nVII, facial motor nucleus. Bar, 100 μm.