FIG. 3.

Cobinding of Cry11A and P20(His₆) proteins to Ni²⁺ column. Crude extracts of cells, one containing Cry11A and the other containing P20(His₆) protein, were mixed and loaded onto Ni²⁺ spin column (Qiagen). The column was washed with buffer containing 20 mM imidazole, followed by elution with the same buffer containing 200 mM imidazole. The Western blot of the “washing” and “elution” fractions was developed with anti-Cry11A primary antibodies.