TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli | ||
| MC1061 | F−lac | 3 |
| BL21(DE3) | F−lac | 3 |
| P. putida PB2442 | trp(r− m+), Rifr Smr | 10 |
| Plasmids | ||
| pQE60 and pQE70 | Apr add six C-terminal His residues to encoded proteins | Qiagen |
| pMMB603 | Apr contains lacIq and cloning cassette of pQE70 | This work |
| pMMB681 | pQE60::NcoI-BglII fragment cry11A(His6) (by PCR) | This work |
| pMMB723 | Smr contains lacIq and cloning cassette of pQE70 | This work |
| pMMB725 | pMMB723::XhoI-HindIII cry4D fragment of pMMB681 | This work |
| pMMB731 | pQE60::NcoI-BglII fragment cry11A+p20(His6) (by PCR) | This work |
| pMMB715 | pMMB603::XhoI-HindIII cry11A(His6) fragment of pMMB681 | This work |
| pMMB736 | pMMB603::XhoI-HindIII cry11A+p20(His6) fragment of pMMB731 | This work |
| pMMB822 | pQE60::NcoI-BglII fragment p20(His6) (by PCR) | This work |
| pMMB823 | pQE60::NcoI-BglII fragment cry11A sequence encoding C-terminal histidines removed as the BglII-HindIII fragment | This work |
| pMMB824 | pT7-7(23) NdeI(blunt-end)-HindIII fragment containing p20 gene under T7 promoter control | This work |