TABLE 2.
Results of bioassays against third-instar Aedes aegypti larvae using recombinant E. coli and P. putida expressing cry11A(His6) or cry11A+p20(His6)
| Strain and protein(s)a | LC50 ± 95% CIb (log10 cells/ml) | Concn range tested (log10 cells/ml) |
|---|---|---|
| E. coli DH5α | ||
| Cry11A | 5.35 ± 5.09 (to 5.55) | 4.52–7.42 |
| Cry11A+P20 | 4.17 ± 2.61 (to 4.74) | 3.18–7.42 |
| Control | NEc | 4.52–7.42 |
| P. putida PB2442 | ||
| Cry11A | NE | 4.52–7.42 |
| Cry11A+P20 | 4.59 ± 2.81 (to 5.33) | 3.18–7.42 |
| Control | NE | 4.52–7.42 |
a
Control, plasmid without gene cluster.
b
CI, confidence interval.
c
NE, no LC50 estimate due to scant mortality.