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. Author manuscript; available in PMC: 2022 Jul 20.
Published in final edited form as: Mol Microbiol. 2013 Nov 27;91(1):121–134. doi: 10.1111/mmi.12448

Figure 2. M. smegmatis mc2155:Δ6129 is resistant to multiple antibiotics but hypersensitive to H2O2.

Figure 2

(a) Ten fold serial dilutions of wild type and mc2155:Δ6129 were spotted on Middlebrook 7H10 containing indicated concentrations of antibiotics. (b) Survival curves of wild type, mc2155:Δ5437 and mc2155:Δ6129 grown for 36 hours in liquid media containing the indicated antibiotics is shown in panels on the right. mc2155:Δ6129 is more resistant to all the drugs tested as compared to the wildtype parent strain. Error bars represent standard deviation of means of three independent experiments. (c) mc2155:Δ6129 shows almost 100% killing when exposed to 32mM H2O2 for 30 mins, a concentration where the viability of wildtype is unchanged. (d) Overexpression of MSMEG_6129 in wildtype cells makes the bacteria hypersensitive to isoniazid similar to mc2155:Δ5437 whereas exogenous expression of MSMEG_6129 in mc2155:Δ6129 restores isoniazid sensitivity to wild type levels.