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. Author manuscript; available in PMC: 2022 Jul 20.
Published in final edited form as: Mol Microbiol. 2013 Nov 27;91(1):121–134. doi: 10.1111/mmi.12448

Figure 5. MSMEG_6129 phosphorylates MSMEG_6127 in vitro.

Figure 5

(a) MSMEG_6127 and (b) M. tuberculosis RsfB purified from E. coli were incubated with purified MSMEG_6129 in kinase buffer containing 1μCi of γ-P32 ATP at 25°C for 30 mins and separated on a 15% SDS-PAGE followed by autoradiography. The positions of MSMEG_6127 and RsfB are marked as well as the 15 kDa and 25 kDa standards. (c) The position of residues phosphorylated in MSMEG_6127 by MSMEG_6129 identified by MALDI TOF/TOF are indicated and include the consensus anti-sigma factor phosphorylation site at position S63.