Table 1. Identification of phosphoproteins absent in mc2155: Δ5437 using MALDI-TOF.
Total cell lysate was prepared from wild type mc2155 and mc2155: Δ5437 were separated by 2-D gel electrophoresis and the phosphorylated proteins identified by fluorescent staining. Differentially phosphorylated proteins were located using a combination of ImageQuant and DeCyder softwares. Of the 51 differentially phosphorylated proteins 21 protein spots were completely absent in mc2155: Δ5437 and were excised from the gel and identified by MALDITOF/TOF (tandem mass spectrometry MS/MS).
Spot # | M. smegmatis ID | M. tuberculosis ID | Protein ID |
---|---|---|---|
4 | MSMEG_0059 | Rv3868 | ATPase, AAA family protein |
9 | MSMEG_1881 | Rv3240c | preprotein translocase subunit SecA |
10 | MSMEG_6091 | Rv3596c | negative regulator of genetic competence ClpC/mecB |
16, 17 | MSMEG_0456 | Rv0006 | DNA gyrase subunit A |
15 | MSMEG_0005 | Rv0005 | DNA gyrase subunit B |
30 | MSMEG_1670 | Rv3318 | succinate dehydrogenase flavoprotein subunit |
33 | MSMEG_6761 | Rv3302c | glycerol-3-phosphate dehydrogenase 2 |
8 | MSMEG_3642 | Rv1832 | glycine dehydrogenase |
45, 46, 47 | MSMEG_2080 | Rv3140 | putative acyl-CoA dehydrogenase |
29 | MSMEG_1807 | Rv3285 | acetyl-/propionyl-coenzyme A carboxylase alpha chain |
7 | MSMEG_2412 | Rv2967c | pyruvate carboxylase |
48 | MSMEG_3046 | Rv1383 | carbamoyl phosphate synthase small subunit |
49 | MSMEG_0702 | Rv0791c | monooxygenase |
50 | MSMEG_2351 | Rv3029c | electron transfer flavoprotein, beta subunit |
51 | MSMEG_6427 | Rv3846 | [Mn] superoxide dismutase |
52 | MSMEG_6129 | - | Putative anti-sigma factor |
43 | MSMEG_1401 | Rv0685 | elongation factor Tu |
6 | MSMEG_3102 | Rv1448c | transaldolase |