Table 1. Identification of phosphoproteins absent in mc²155: Δ5437 using MALDI-TOF.

Total cell lysate was prepared from wild type mc²155 and mc²155: Δ5437 were separated by 2-D gel electrophoresis and the phosphorylated proteins identified by fluorescent staining. Differentially phosphorylated proteins were located using a combination of ImageQuant and DeCyder softwares. Of the 51 differentially phosphorylated proteins 21 protein spots were completely absent in mc²155: Δ5437 and were excised from the gel and identified by MALDITOF/TOF (tandem mass spectrometry MS/MS).

Spot #	M. smegmatis ID	M. tuberculosis ID	Protein ID
4	MSMEG_0059	Rv3868	ATPase, AAA family protein
9	MSMEG_1881	Rv3240c	preprotein translocase subunit SecA
10	MSMEG_6091	Rv3596c	negative regulator of genetic competence ClpC/mecB
16, 17	MSMEG_0456	Rv0006	DNA gyrase subunit A
15	MSMEG_0005	Rv0005	DNA gyrase subunit B
30	MSMEG_1670	Rv3318	succinate dehydrogenase flavoprotein subunit
33	MSMEG_6761	Rv3302c	glycerol-3-phosphate dehydrogenase 2
8	MSMEG_3642	Rv1832	glycine dehydrogenase
45, 46, 47	MSMEG_2080	Rv3140	putative acyl-CoA dehydrogenase
29	MSMEG_1807	Rv3285	acetyl-/propionyl-coenzyme A carboxylase alpha chain
7	MSMEG_2412	Rv2967c	pyruvate carboxylase
48	MSMEG_3046	Rv1383	carbamoyl phosphate synthase small subunit
49	MSMEG_0702	Rv0791c	monooxygenase
50	MSMEG_2351	Rv3029c	electron transfer flavoprotein, beta subunit
51	MSMEG_6427	Rv3846	[Mn] superoxide dismutase
52	MSMEG_6129	-	Putative anti-sigma factor
43	MSMEG_1401	Rv0685	elongation factor Tu
6	MSMEG_3102	Rv1448c	transaldolase