(See Supporting Movies 2, 3, and 4) Three-dimensional
graphene reconstruction through PC-TFC and AM-TFC methods in three
NIH-3T3 cells after 24 h treatment with nGO. (a,f,k) ROIs containing
the NIH-3T3 cells in the recorded digital holograms in which the accumulation
of internalized nGO has a disruptive effect on the interference fringe
pattern (blue insert). (b,g,l) Numerically retrieved QPMs with highlights
in red (inset), the dark spot due to graphene disturbance in the recorded
holograms in (a,f,k). (c,h,m) Isolevel representation of the 3D PC-TFC
reconstructions. In (c,h), the nGO accumulation (black) is segmented
and isolated from the outer cell (red), while this is not possible
in (m). (d,i,n) Numerically retrieved AMs, with highlights in green
(inset), the dark spot due to graphene disturbance in the recorded
holograms in (a,f,k). (e,j,o) Isolevel representation of the 3D AM-TFC
reconstructions in which the nGO accumulation (black) is segmented
and isolated from the outer cell (green). Scale bars are 10 μm.
The intervals highlighted in the colorbars refer to the insets.