ζ time series for (a) unbiased MD, and (b) SPIB augmented metadynamics. Multiple back and forth transitions were captured in SPIB metadynamics compared to unbiased MD. (c) Free energy along OP ζ highlighting R,rlrrl, llrlr, L metastable states showing similar free energy for L and R, (d) free energy along 2-d RC for time delay, Δt = 0.3ns, (e) converged state labels, and (f) previously used27 expertise-based RC ζ projected on the 2-d RC generated by SPIB.