FIG. 2.
Determination of REOL3 primer set RT-PCR detection sensitivity for reovirus type 1. Lanes 1 to 14, RT-PCR products obtained from 10−2 to 10−8 dilutions (in duplicate), respectively, of purified RNA of the reovirus type 1 stock; lane 15, no-template control; lanes M, 2,000- to 50-bp DNA molecular weight marker XIII. The lowest dilution at which virus was detected was also the 10−6 dilution for reovirus types 2 and 3 (data not shown). This was equivalent to approximately 3, 30, and 0.3 PFU of reovirus types 1, 2, and 3 per reaction mixture, respectively.