Fig. 1.

Robust, transient tau hyperphosphorylation after 2-h exposure to isoflurane without temperature-control. Total (DA31) and phosphorylated tau (ptau) in soluble fractions were quantified with ELISA, and the ptau/total tau ratios were compared. A, B In male 5-month-old P301L mice (n per group = 8, 8, 8/7), we found hyperphosphorylation at the CP13 (Ser202) and PHF1 (Ser396/404) epitopes in the cortex (A) and hippocampus (B) immediately after anesthesia. C, D Likewise, in male 4-month-old COMTKO/P301L mice (n per group = 8, 10, 8), we found hyperphosphorylation at the CP13 and PHF1 epitopes in the cortex (C) and hippocampus (D) immediately after anesthesia. In both models, hyperphosphorylation returned to the level of controls 24 h after anesthesia. Data are mean ± SEM. One-way ANOVA with Tukey’s post-hoc test was performed. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. C, Controls; AN, Anesthesia; 24H, 24-h post-anesthesia