Fig. 4.

Hyperphosphorylation of soluble and insoluble tau 3 months after anesthesia in COMTKO/P301L mice. Total (DA31) and phosphorylated tau (ptau) were quantified with ELISA, and the ptau/total tau ratios were compared. A, B In the soluble fraction of mice that were anesthetized when 4-month-old, no differences were found in the cortex (A), but phosphorylation at the CP13 epitope was increased in the hippocampus (B). C In the insoluble fraction of the cortex, phosphorylation was increased at all three phospho-epitopes. n per group = 17, 13. Data are mean ± SEM. Student’s t-test was performed. *p < 0.05, ****p < 0.0001. C Controls, 3 M 3-months post-anesthesia, mo month-old