Skip to main content
. Author manuscript; available in PMC: 2023 Jun 15.
Published in final edited form as: Bioconjug Chem. 2022 May 11;33(6):1179–1191. doi: 10.1021/acs.bioconjchem.2c00142

Scheme 4. Single-Step Transglycosylation of Drug-Oxazoline Conjugates to Make ADCs (33–35)a.

Scheme 4.

aThe reactions were performed in a PBS buffer with 5% DMSO, and the antibody concentration was 20 mg/mL (isolated yields after protein A purification). For each reaction site: (a) 15 equiv of oxazoline was used; (b) 25 equiv of oxazoline was added in two portions.