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. 2022 Jul 20;13:330. doi: 10.1186/s13287-022-03010-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — MSC-EXO induced the remodeling of macrophage phenotype in vitro. A Reparative confocal microscopy images showing colocalization of PKH26-labelled EXO with macrophage cell line RAW264.7. Scale bar = 50 μm. B RT-PCR analyzed the mRNA level of M1 and M2 markers of M1 + PBS (RAW264.7 + LPS/IFNγ + PBS) and M1 + EXO (RAW264.7 + LPS/IFNγ + EXO) groups. C–D The protein level of iNOS and Arg1 was measured by western blot, and quantification of the relative expression in the bands in different groups was calculated by Image-Pro Plus. E, F Reparative flow cytometry plots showing the percentage of CD86 (M1 marker) and CD206 (M2 marker) in different groups and the percentage of CD86 + or CD206 + cells was analyzed by GraphPad Prism (n = 4). G Reparative confocal microscopy images showing colocalization of PKH26-labelled EXO with BMDM. Scale bar = 20 μm. H, I The protein level of iNOS and Arg1 was measured by western blot and quantification of the relative expression in the bands in different groups was calculated by Image-Pro Plus. J RT-PCR analyzed the mRNA level of M1 and M2 markers of B1 + PBS (BMDM + LPS/IFNγ + PBS) and B1 + EXO (BMDM + LPS/IFNγ + EXO) groups