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. 2022 Jul 20;21:149. doi: 10.1186/s12943-022-01619-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — FTO mediates m⁶A modification of circGPR137B to form a positive feedback loop with circGPR137B. MeRIP (A) and m⁶A dot blot (B) analyses of the effects of FTO on the total m⁶A levels in HepG2 and Hep3B cells. C MeRIP analysis of the effect of FTO on the m⁶A levels of circGPR137B in HepG2 and Hep3B cells. RT-qPCR (D) and western blot (E) analysis of the effect of FTO overexpression or knockdown on the RNA levels of circGPR137B in HCC cells. RT-qPCR (F) and western blot (G) analysis of the effect of circGPR137B knockdown or miR-4739 inhibitor on FTO expression in SK-hep-1 cells. H RIP analysis of the binding between FTO and circGPR137B in HepG2 and Hep3B cells. MTT (I) and transwell (J) analysis of the cell proliferation and invasion after transfection with FTO and si-circGPR137B plasmids in HepG2 and Hep3B cells. Data are the means ± SEM of three experiments. *P < 0.05, **P < 0.01