FIG 1.

Overview of methods for performance studies. Time estimates included in brackets are based on runs containing 24 samples and reported by those performing the assay steps for this study. Each specimen underwent extraction with or without bead beating prior to the combination of eluates. Eluates from each specimen were processed with the metagenomic and RPIP targeted workflows. Data from each workflow were evaluated using the same conditional reporting guidelines and compared to composite clinical standard results obtained for the specimen. Identical processing and NGS workflows were utilized to establish analytical sensitivity using spiked samples, with comparisons being made to the organism pools rather than a composite clinical standard.