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. 2022 Aug;28(8):1074–1088. doi: 10.1261/rna.079206.122

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© 2022 Woodside et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

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FIGURE 4. — Type III-A crRNPs can effectively target multiple mRNA transcripts simultaneously. (A–C) Expression of the L. lactis type III-A crRNPs with single or multiple crRNAs was induced, and northern analysis was performed with probes against lpp mRNA (A), cspE mRNA (B), ompF mRNA (C), or 5S rRNA (A–C). The positions of full-length mRNAs (dot) and expected cleavage products (red star) are indicated in each panel. The sizes of the molecular weight markers (M) are indicated in each panel. The relative positions of the crRNA and the northern probe for each mRNA are shown below each panel.