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Strategies for the preparation of covalent NA‐protein bioconjugates. a) Conjugation strategy based on the introduction of handles for bioorthogonal ligation reactions. b) Conjugation of ONs and native proteins by heterobifunctional linkers. c) Examples of heterobifunctional linkers bearing various spacers commonly used for the DNA‐protein bioconjugation. d) Conjugation method with novel BA‐PFP reagent 2 described in this work.
