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. 2021 Nov 3;60(49):25905–25913. doi: 10.1002/anie.202109713

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© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Optimization of the LC‐MS method for the characterization of the model DNA‐antibody conjugate. a) Conditions used for the preparation of anti‐nucleoporin nanobody conjugates with a short ss11‐mer DNA ON. b) Comparison of the effect of different LC mobile phase additives on the ion series and deconvoluted mass spectra for conjugate 594Nup98 G85C‐ss11‐mer. c) LC‐MS characterization of prepared ss11‐mer conjugates of different anti‐nucleoporin nanobody variants with deconvoluted mass spectra and masses that correspond to expected products. For full LC‐MS spectra see Figures S30–S40.