Figure 4.

Site‐specific NA‐antibody conjugates. a) Scope of the conjugation of BA‐modified ONs through cysteine residues to proteins and antibodies of various sizes and formats. b) Deconvoluted mass spectra obtained from the modification of four model proteins with the 5′‐BA‐ss11‐mer ON. c) Deconvoluted mass spectra obtained from the modification of C2Am‐C95 with different BA‐modified ONs. d) Deconvoluted mass spectra of prepared DNA‐antibody conjugates that bear antibodies against HER2 or CD33 receptors. To observe individual LC and HC of IgG mAbs, disulfide bonds between LCs and HCs were reduced prior to LC‐MS analysis (SI). e) Deconvoluted mass spectra of four different 2Rb17c‐C138 conjugates. Each DNA‐protein bioconjugation reaction was performed under the optimized conditions (SI) at least two times and led to identical, pure products. For full LC‐MS spectra see Figures S41, S43–S52, S54, S56–59.