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. 2021 Oct 25;119(1):162–175. doi: 10.1002/bit.27961

Figure 4.

Figure 4

Comparison of antibody–drug conjugates (ADCs) produced in cell free protein synthesis using batch and continuous extract. Extract was made using either a batch fermentation or a continuous fermentation from strain SBDG299. (a) DBCO‐maytansine warhead used to arm aCD74 ADC. (b) ADCs conjugated from mAbs produced with batch extract or two different lots of continuous extract were used for in vitro cell binding with the indicated cell lines. (c) Cell killing of ADCs in (b) with the indicated cells lines. SUDHL6 and MINO cells are CD74 positive and OPM2 cells are CD74 negative. aGFP ADC is an isotype control ADC generated from an IgG whose variable regions target the protein GFP