Table 2.

Media for continuous fermentation

Reagent	Reaction Concentration	Unit
10× step 1
Potassium phosphate monobasic	30	g/L
Potassium phosphate dibasic	45.8	g/L
Ammonium sulfate	15	g/L
Sodium citrate dihydrate	12.75	g/L
Potassium chloride	10	g/L
Trace metals	3	ml/L
10× step 2
Potassium hydroxide	96.5	g/L
l‐Asparagine·H2O	1.8545	g/L
Glycine	3.3772	g/L
l‐Histidine	0.5803	g/L
l‐Isoleucine	1.6638	g/L
l‐Leucine	1.9327	g/L
l‐Lysine·HCl	2.3865	g/L
l‐Methionine	0.8874	g/L
l‐Phenylalanine	0.9065	g/L
l‐Proline	2.2071	g/L
l‐Threonine	2.2765	g/L
l‐Tryptophan	0.5652	g/L
l‐Tyrosine	0.9772	g/L
l‐Valine	1.0643	g/L
Betaine·HCl	2.0084	g/L
Choline chloride	0.028638	g/L
Nicotinic acid (niacin)	0.02512	g/L
p‐aminobenzoic acid	0.025600	g/L
Calcium pantothenate	0.009397	g/L
Pyridoxine (B6) (HCl)	0.001471	g/L
Riboflavin (B2)	0.003886	g/L
Thiamine (B1) (HCl)	0.01766	g/L
Biotin (vitamin H)	0.11418	mg/L
Cyanocobalamin (vitamin B12)	0.00858	mg/L
Folic acid	0.06534	mg/L
1×80/80 media
10× step 1	100	ml/L
10× step 2	100	ml/L
50% glucose	10	ml/L
Sulfuric acid	3.4	ml/L
1.31 M magnesium sulfate heptahydrate	2.4	ml/L
50% antifoam 204	0.909	ml/L